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The resulting free fatty acids exit the fat cell through fatty acid transport proteins and enter the bloodstream

For example, the GST enzymes, part of phase II detoxification, are upregulated by the Nrf2 pathway.[ref][ref] Direct antioxidant effects: In addition to activating endogenous antioxidants via the Nrf2 pathway, sulforaphane also has some direct antioxidant properties, as it can scavenge free radicals and reactive oxygen species (ROS)

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Anesthesia was administered prior to drug injections to ensure animal comfort and minimize stress