Laboratories using the compound in time-course or dose-response work should prepare fresh aliquots where possible, recording reconstitution date and storage temperature alongside each experimental block
Briefly, 400 ]x slices were cultured on (Miiipore, Billerica, MA) for 3 days after which they were biolistically transfected with tomato fluorescent protein (TFP) using a Helios Gen Gun (BioRad, Hercules, CA), according to the manufacturer's protocol, to visualize dendritic arbors
Some patients experience headache, others may feel nauseated
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