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NNMT (nicotinamide N-methyltransferase) is the enzyme 5-Amino-1MQ inhibits, and tissue expression varies significantly across organs: adipose tissue shows 812 higher NNMT activity than skeletal muscle, which is why subcutaneous injection near adipose depots produces localised effects rodent models couldn't predict

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Cagrilintide is studied in vitro for its affinity across the amylin receptor family (AMY1R, AMY2R, AMY3R) and its calcitonin-receptor cross-reactivity