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During this test the surgeon presses the center of the disk with the probe

Switching up where you inject helps avoid soreness and ensures the shot works well

Nugget01 GLP-1 Novice No Source Discussion #2 This chart is pretty accurate

For tonic NMDAR-mediated current recording, neurons were held at + 40 mV in normal ACSF with internal solution containing (in mM): 110 Cs methylsulfate, 20 TEA-Cl, 15 CsCl, 4 ATP-Mg, 0.3 Na3-GTP, 0.5 EGTA, 10 HEPES, 4.0 QX-314, and 1.0 spermine, pH adjusted to 7.2 with CsOH, and osmolality set to 290300 mOsm/kg with sucrose