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The enzyme aromatase is widely expressed in the forebrain of primates (including humans), and although estrogens are more associated with female functions, the expression of this enzyme in the CNS does not depend on plasma E2 levels (135, 136)
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The hydroxyl radical (OH ) is the most damaging member as it has the ability to trigger a cyclic chain reaction and cause further peroxidation of other PUFAs
The media were replaced daily, until day 6 when culture media were changed to neuronal media containing Neurobasal-A (Gibco/Fisher, Cat# 10-888-022) supplemented with 2% Gibco B-27 serum-free supplement without vitamin A (Fisher Scientific, Cat# 12-587-010), 1% GlutaMAX Supplement (Gibco/Fisher, Cat# 35-050-061), and 1% penicillin/streptomycin (Gibco/Fisher Scientific, Cat# 15-140-122)
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