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The mixture was then centrifuged at 10,000 g at room temperature for 10 min, and supernatant was used to detect absorbance at 532 and 600 nm using a microplate reader (BioTek)

Guarana is derived from seeds of the plant, Paullinia cupana, a native plant to the Amazon basin

Finally, N-acetyl-cysteine (NAC) has been shown to partially prevent tolerance development to nitrate-induced antianginal effects

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