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The heterogeneity of zones allows cells to perform specific functions effectively in response to various injuries

The protein pellet was resuspended in 100 L RIPA buffer (10 mM Tris-HCl, pH 8.0, 1 mM EDTA, 0.5 mM EGTA, 1% Triton X-100, 0.1% Sodium Deoxycholate, 0.1% SDS, and 140 mM NaCl) to normalize the intensity values for each targeted metabolite

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