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WS diluent: 10 mg/ml BSA, 140 mM NaCl, 1.2 mM MgSO4, 1 mM CaCl2, 0.5 mM DTT, 1 mM glucose, 15 mM HEPES-NaOH, pH 7.4 3

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Herold KC, Hagopian W, Auger JA et al (2002) Anti-CD3 monoclonal antibody in new-onset type 1 diabetes mellitus

Thus, carnosine readily forms a charge-transfer complex with oxygen free radicals (e.g., superoxide radical, hydroxyl radicals, peroxyl radicals, and nitric oxide), non-radical ROS (e.g., peroxynitrite, hypochlorous acid, singlet oxygen, and H 2 O 2 ), and deleterious aldehydes (e.g., malondialdehyde and formaldehyde) to confer anti-oxidative effect and protect cell membrane and intracellular organelles (e.g., mitochondria) from damage (Kohen et al