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The eluent was prepared as follows: Eluent A was prepared by adding 15 mL of 500 mM 1-octanesulfonic acid sodium salt solution and 0.2 mL of trifluoroacetic acid to an appropriate volume of distilled water for HPLC (Nacalai Tesque), made up to a total of 1000 mL

The form of berberine is also a consideration

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