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Experiments were carried out in triplicate

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Current research into their roles in DNA methylation, histone modification, or microRNA regulation is limited, and their contribution to CRC chemoprevention appears to be of secondary importance at this time

The mobile phase A (H 2 O/0.1% formic acid) and mobile phase B (100% acetonitrile/0.1% formic acid) were delivered through a liquid chromatograph nanoElute (Bruker Daltonics GmbH, Bremen, Germany) at a flow rate of 200 nL/min, maintaining the column temperature at 50C