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Cigarette smoke exposure HBE cells were exposed to mainstream smoke from 3R4F University of Kentucky reference cigarettes (Lexington, KY) using a VC-10 Smoking Robot (Vitrocell, Waldkirch, Germany) in accordance with ISO 3308 standards (puff duration 2 s, hold time 0 s, exhaust time 8 s, puff volume 35 mL, frequency 60 s, 6 puffs per cigarette)

But Gilbert never found a company willing to mass-produce his invention, and therein lies a classic American tale of an inspired tinkerer way ahead of his time: 55 years later, e-cigarettes are a $10 billion industry worldwide

In microeconomics, two products may be substitutes if one product replaces the other in use as a result of changed conditions (e.g