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Description

6 h later cells were collected, and directly lysed using RIPA buffer (150 mM NaCl, 50 mM Tris-HCl [pH 8.0], 5 mM EDTA pH 8.0, 0.5% NP-40, 0.5% Triton X-100, 0.5% sodium deoxycholate, 0.1% SDS, 1 cOmplete Mini protease inhibitor cocktail (Roche), 1 PhosSTOP phosphatase inhibitor cocktail (Roche), 10 M MG132)

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Cell 174, 13881405.e21

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keep dry and protected from light

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b-c , SDS-PAGE gel of His-tagged GPX4 U46C and GPX4 U46C_R152H as stained by Coomasie Blue

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