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Elevated homocysteine and methylmalonic acid (MMA) are biomarkers of functional B12 insufficiency

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While in vitro models allow more controlled and complex study designs compared with in vivo models, cultured muscle cell models typically have low endogenous GLUT4 expression [115,116], often lack a mature transverse tubule system [117], and do not recapitulate in vivo insulin responses to the same degree as the 3T3-L1 adipocyte