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Total Peptide: 1000mcg Total Liquid Volume: 2ml (which is 200 units on a U-100 syringe) Now the calculation is: 1000mcg / 200 units = 5mcg per unit In this case: A 20mcg dose would require 4 units

Nanoparticles were diluted in phenol red-free DMEM/HAMs F-12 medium (Gibco, Life Technologies, Carlsbad, CA, USA) supplemented with 5% FBS at a final concentration of 100 g/ml and added to the donor compartment (0.2 ml)

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Phase 2 dose escalation protocol The Phase 2 trial evaluated multiple dose levels ranging from 0.5mg to 12mg administered once weekly via subcutaneous injection