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The variable of exposure was detailed e-cigarette usage that was recorded through standardized questionnaires, including duration, frequency per day, preferred device type, and nicotine concentration level (self-reported based on product packaging information)

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Supernatant was diluted 1:1 with mobile phase A (see below) prior to injection of 25 L into the LCMS/MS system (LC: Prominence series from Shimadzu, Kyoto, Japan

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