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Dilute this further to produce 18.4% and 13.8% (w/v) iodixanol solutions with a 0.75% (w/v) NaCl solution in 10 mM Tricine-NaOH, pH 7.0 Rabbit PMNs have also been isolated from an LRP using a two-layer iodixanol gradient (density 1.085 and 1.086 g/ml) with centrifugation at 500 g for 35 min [28,29] 4

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