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Acknowledgments The authors would like to recognize and thank Vince Abushaban, David Caprio, David Goldstrohm, Jenai Kailey, Dan LoTurco, Gates Roe, Larry Toft, Karen Trembler, and Dong Wang for their technical support

For the non-interventional cohort, a group size of 20 subjects will detect a minimum difference in baseline GSH of at least 16% between young and elderly healthy subjects (0.05 alpha, 0.8 power) based on an inter-subject CV of 22%

The blue color is from the copper(II) ion chelated by the GHK ligand this is normal and confirms intact complex formation

Mark Hyman, MD This is a valuable clarification