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Funding Statement This paper was not funded, but it was supported by the National Institute for Health and Care Research (NIHR) Leicester Biomedical Research Centre

Put 5 l of the mixture on a glass slide, drop it in 95% ethanol for 30 s, then add 15 l of FITC-PNA solution, react at 4C for 30 min, and then remove it by PBS ( Antioxidant Indexes Reactive oxygen species kit (Purchased from Biyuntian Biological Company), malondialdehyde (MDA) kit (Purchased from Soleibao Biological Company), superoxide dismutase (SOD) kit (Purchased from Soleibao Biological Company), glutathione peroxidase (GSH-PX) kit (Purchased from Biyuntian Biological Company) were used to detect various antioxidant indicators of thawed sperm

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