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A wide range of prior efforts have focused on creative ways to measure the accumulation of small molecules, including coupling of periplasmic accumulation to b-lactamase activity 79 , split luciferase 14 , modification of antibiotics with fluorescent handles 80 , LC-MS-based quantification 15,41,42,81,82 , electrophysiology using planar lipid bilayers 83,84 , and microfluorospectrometry 85,86,87

They handle short room-temperature periods during shipping, keep well in the fridge, and stay stable longest frozen at -20C or colder

Galvan-Roman J