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Supplementary Table 1) was mixed with 3 l of transfection reagent (DharmaFECT 1, Horizon Discovery) in a total volume of 500 l of OptiMEM (Gibco) and incubated for 20 minutes at room temperature

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FIGURE 2 IGF SIGNALING IN THE CONTEXT OF LIGAND-RECEPTOR INTERACTIONS The IGF system is comprised of type I and type II receptors, their ligands (IGF-1 and IGF-2), IGFBPs, and IGFBP proteases (Le Roith et al., 2001)