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Membranes were blocked with 5% NFDM in Tris Buffered Saline with 0.2% Tween-20 (TBST) for 2 h at room temperature followed by primary antibody incubation overnight at 4 C in 5% NFDM in TBST (1:5000 596 CFTR antibody that recognizes CFTR residues 12041211

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