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Considering that the human APOE isoform further regulates A clearance efficiency, and that sex and age modulate APOE-related AD risk via hormonal and aging factors (Cacciaglia et al., 2022), APOE-4 is associated with more severe cognitive decline and increased AD risk, particularly in individuals aged 7080 (Cacciaglia et al., 2022)

The Boc-protected cystine-1,1- 13 C was reacted with two equivalents of glycine tert -butyl ester (OtBu) hydrochloride using 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide monohydrochloride and 1-hydroxybenzotriazole to produce the N and C-protected dipeptidyl (Boc-Cys-Gly-OtBu) disulfide derivative