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Notably, by day 3, assembloids reproducibly established key periportal architectural features, with cholangiocytes (KRT19 + nuclear green fluorescent protein (nGFP) + ) forming bile duct-like structures containing open lumina in close proximity to portal fibroblasts (nuclear red fluorescent protein (nRFP)) and both cell types embedded within the hepatocyte (HNF4A + ) parenchyma

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