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where they have enriched monocytes from healthy volunteers to generate the functional genomics datasets reanalyzed herein ( Cell Culture Monocytes were cultured in complete media consisting of Dulbeccos Modified Eagle Medium containing high glucose and sodium pyruvate (ThermoFisher Scientific) with 10% heat-inactivated fetal bovine serum (Wisent BioProducts) and 100U/mL penicillin/streptomycin (Corning)

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He also flags that glutathione contains a thiol group that oxidizes in water over time, converting the active form to an inactive one, and recommends using reconstituted vials within 14 days rather than the 28-day window common with other peptides