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Operating Room (O.R.) and Non-O.R

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In mice, CHAC1/BOTCH promotes neuronal differentiation by inhibiting NOTCH1 and both its depletion and overproduction severely disrupt neurogenesis ( CHAC1/BOTCH in zebrafish leads to severe defects in brain, heart and myotome development, resulting in embryonic lethality, highlighting its conserved and essential role (Yadav et al., 2019)

Exclusion criteria: (1) Patients with abnormal coagulation function [limits: prothrombin time (PT) between 1014 seconds, activated partial thromboplastin time (APTT) between 2540 seconds, and fibrinogen (Fib) levels between 2.04.0 g/L] and immunodeficiency [either primary immunodeficiency (congenital or hereditary conditions leading to impaired immune responses) or secondary immunodeficiency (conditions acquired through HIV infection, chronic immunosuppressive therapy, or severe malnutrition)]