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Critical Points: Lyophilization must be done carefully to avoid damaging liposome structure or cargo

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10.3390/toxins11040191 87 GajskiG.imbora-ZovkoT.RakS.OsmakM.Garaj-VrhovacV

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Detergent was removed by adding Bio-Beads SM-2 (100 mg/ml) (Bio-Rad, 1523920) and rotating for 2 hours at room temperature followed by five additional rounds at 4C every two hours

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Thawing : Thaw the aliquots on ice or in a cold room

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