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The safest reconstitution method involves: (1) Using sterile technique (clean workspace, alcohol swabs, sterile syringes), (2) Using bacteriostatic water for injectionnever tap water, distilled water, or saline, (3) Allowing peptide vials to equilibrate to room temperature before reconstituting, (4) Injecting bacteriostatic water slowly down the vial wall rather than directly onto the powder, (5) Avoiding shaking or vigorous agitation, and (6) Storing reconstituted peptides refrigerated (2-8C) and protected from light

Hanger DP, Anderton BH, Noble W

Meister A, Anderson ME (1983)

However, APR-246 also has p53-independent function via MQ binding to thioredoxin reductase and glutathione, leading to depletion of glutathione and accumulation of reactive oxygen species (ROS), which can feed forward p53 activation (Figure 1).1918 Indeed, the authors also show synergy in TP53 knockout mutant cell lines where there is absence of p53, albeit with less synergy than in the missense mutant model