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Future progress will require: (1) Parameter optimization studies: Systematic in vitro screening of DECLARATIONS Authors contribution Writing - original draft, investigation, conceptualization, and funding acquisition: Wang LY Writing - review and editing: Fan MY, Jiang XY, Bing KJ, Wang YJ, Zhang H Writing - review and editing, supervision: Wang KS, Huang YM Availability of data and materials Not applicable

Gut Permeability, Inflammation, and DAO Deficiency Gut permeability and inflammation can impact DAO (diamine oxidase) production and function, although they may not necessarily lead directly to a deficiency in DAO due to an excessive need for histamine metabolism

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Glucuronidation (UGT Enzymes) Glucuronidation is the most common Phase II conjugation pathway, accounting for roughly 35% of all drug conjugation reactions