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BPC-157 has extensive research support for promoting gut tissue healing, keeping gut barrier integrity, and protecting against many forms of gut damage

Cells were lysed for 15 minutes in ice-cold RIPA buffer consisting of 1% NP40, 0.1% SDS, 0.5% deoxycholate in PBS, supplemented with 1 mM PMSF, 1X cOmplete, EDTA-free Mini Protease Inhibitor Cocktail (Sigma Cat#11873580001) and 1X PhosSTOP (Sigma Cat#4906845001)

Funding This research was supported by the National Natural Science Foundation of China (82371382, 81771381), Anhui Provincial Key Research and Development Project (2022e07020030, 2022e07020032), Science Research Project of Bengbu Medical College (2021byfy002), Anhui Province Key Laboratory of Tissue Transplantation (Bengbu Medical College)(AHTT2022B001), and the Undergraduate Innovative Training Program of China (202110367037)