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Then, 180 l of reaction mix (100 mM citratephosphate buffer pH 4.0 and 1 mM ABTS) were added using a Multidrop robot (Multidrop Combi, Thermo Scientific) and laccase activity was measured at room temperature in a plate reader SpectraMax ABS Plus (Molecular Devices) at 418 nm

Diabetologia (2020) 63:267588

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However, steaming or blanching these foods seems to help break down some of the enzymes responsible for the goitrogen effect, making them safer for your thyroid health