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Conclusion Oxidized glutathione is an important intermediate in the redox cycle of glutathione

The supernatant was carefully discarded, pellets (16) were combined in cold PBS or radioimmunoprecipitation assay (RIPA) with protease and phosphatase inhibitors (P0044-1ML, Sigma-Aldrich, and A32953, Thermo Scientific) at a pre-determined volume as was appropriate for the experiment planned and stored in 80 C until subsequent experimentation