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Samples and standards were diluted fourfold in sample dilution and plates were processed according to the manufacturers instructions

Monitoring patients during treatment is crucial to ensure safety and efficacy

Because MOTS-c enhances insulin-independent glucose uptake, patients with type 1 diabetes or those using exogenous insulin should be monitored carefully to prevent hypoglycemia, though this theoretical risk has not materialized in clinical reports to date

Any mention of published preclinical data, mechanisms of action, or experimental outcomes is provided exclusively for scientific context and literature review