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SLC7A11 is a transmembrane protein with 12 transmembrane domains, with both its N- and C-termini located intracellularly ( Figure 2 )

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Without adequate glutathione to support detoxification, these toxic Phase I intermediates would accumulate, causing massive oxidative stress, mitochondrial dysfunction, and ultimately, severe liver damage

Western blot analysis involved preparing whole cell lysates using RIPA lysis buffer, quantifying protein concentration via BCA assay, followed by SDS-PAGE, transfer to nitrocellulose membranes, incubation with primary and HRP-conjugated secondary antibodies, and detection using an ECL solution [1]