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The best binding was observed for the histidine containing peptide as expected, whereas slightly weaker affinity was observed for amide (Q, N) or amine (K) containing side chain, whereas carboxy (D,E), thioether (M) or aromatic (F,Y,W) functional groups hardly enhanced affinity compared to the single histidine containing peptide (Fig

Jun 15 2011;83(12):1425-1430

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This test allows the doctor to determine the size of the gland and identify the location and degree of the obstruction