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10.12729/jbr.2012.13.1.65 45 ChenM.Aoki-UtsuboC.KameokaM.DengL.TeradaY.KamitaniW.et al (2017)

Common Causes of Peptide Degradation Many different processes and factors can affect peptides: Peptide hydrolysis caused by moisture exposure Oxidation resulting from oxygen and exposure to light Changes in temperature Bacterial degradation in peptide solutions Contamination during laboratory handling Repeated freeze-thaw cycles (Sigma-Aldrich, 2023a

Then, 100 L mixed internal standard solution and 400 L methanol solution (precooled at -20C) were added to each sample and vortexed thoroughly for 60 s

Cagrilintide (an amylin analog) signals brain regions involved in satiety